Holistic Quality Assessment of Traditional Smoked Fish from the Sangihe Islands: Proximate, Sensory, and Microbiological Profiles

Authors

  • Eko Cahyono Politeknik Negeri Nusa Utara
  • Frans Gruber Ijong Politeknik Negeri Nusa Utara https://orcid.org/0000-0001-5828-0795
  • Yana Sambeka Politeknik Negeri Nusa Utara
  • Wendy Alexander Tanod Politeknik Negeri Nusa Utara
  • Frets Jonas Rieuwpassa Politeknik Negeri Nusa Utara
  • Novalina Maya Sari Ansar Politeknik Negeri Nusa Utara
  • Yosephina Margaretha Jawa Batafor Institut Keguruan dan Teknologi Larantuka

DOI:

https://doi.org/10.36568/jone.v4i3.743

Keywords:

food microbiology, hedonic test, proximate analysis, sangihe, sensory quality, traditional smoked fish

Abstract

This study characterized the chemical, sensory, and microbiological properties of four smoked fish products typical of the Sangihe Islands (pinekuhe, skipjack, tinapa, and roa). Twelve samples were collected from four production centers (n=3 per smoked fish type). Proximate analysis (moisture, ash, fat, protein, and carbohydrates) followed SNI standards; sensory evaluation involved 30 panelists using a 1–9 hedonic scale; and microbiological quality was measured via Total Plate Count (TPC). Data were analyzed using one-way ANOVA and Tukey’s post-hoc test. Significant differences were found among products for all proximate parameters (p<0.01; η²=0.72–0.89). Smoked roa fish had the lowest moisture content (17.46±0.31%) and the highest protein (23.78±0.16%) and ash (4.15±0.06%) contents. Smoked tinapa had the highest moisture content and was more prone to spoilage. Smoked skipjack had the highest fat content and the best appearance and texture scores, while smoked pinekuhe received the highest flavor score. All samples met the maximum ALT limit according to SNI (5.0×10⁵ CFU/g). Each product has specific quality advantages; however, products with relatively high moisture content require better hygiene practices to improve safety and shelf life. These findings provide an initial scientific basis for efforts to improve the quality of local products in the Sangihe Islands and surrounding area.

References

Kwaghvihi O, Akombo P, Omeji S. Effect of Wood Smoke on the Quality of Smoked Fish. 2020;04:72-82. doi:10.46382/mjbas.2020.4207

Meyer-Rochow V. A Review of Traditional, Time-Honoured Foods and Recipes: To Choose to Use or Not to Use. Foods. 2025;14. doi:10.3390/foods14193371

Suherman A, Hernuryadin Y, Suadela P, Furkon UA, Amboro T. Transformation of Indonesian capture fisheries governance: Review and prospects. Mar Policy. Published online 2025. doi:10.1016/j.marpol.2025.106619

GarridoGamarro E, Svanevik C, Lundebye A, et al. Challenges in the implementation of Food Safety and Quality Assurance Systems in Small-Scale Fisheries. Food Quality and Safety. Published online 2023. doi:10.1093/fqsafe/fyad007

Ansar N, Ijong F, Tanod W, et al. Quality Of Malalugis Salted Fish: A Traditional Product From Bebalang, Sangihe Islands. Chanos Chanos. Published online 2024. doi:10.15578/chanos.v22i1.14303

Rieuwpassa F, Tapotubun A, Matrutty T, Nanlohy H, Savitri IKE. Nutritional composition of smoked skipjack produced in Ambon. In: IOP Conference Series: Earth and Environmental Science. Vol 1207. 2023. doi:10.1088/1755-1315/1207/1/012010

Karimela E, Ijong F, Dien H. Characteristics of Staphylococcus aureus Isolated Smoked Fish Pinekuhe from Traditionally Processed from Sangihe District. 2017;20:188-198. doi:10.17844/JPHPI.V20I1.16506

Haurissa ER, Yunita M, Sulfiana. Total Plate Count Analysis and Food-Contaminating Bacterial Identification of Smoked Tatihu (Thunnus albacares) Sold in Several Traditional Markets in Ambon, Indonesia. Folia Medica Indonesiana. Published online 2024. doi:10.20473/fmi.v60i3.62231

Palawe J, Suwetja I, Mandey L. KARAKTERISTIK MUTU MIKROBIOLOGIS IKAN PINEKUHE KABUPATEN KEPULAUAN SANGIHE [Microbiological Quality Characteristics Of Fish Pinekuhe From Sangihe Regency]. Published online 2014. https://consensus.app/papers/karakteristik-mutu-mikrobiologis-ikan-pinekuhe-palawe-suwetja/6d6d7c593fb65a57aad1194293b8ed84/

Pop MD. Sensory Evaluation Techniques of Food. Annals of “Valahia” University of Târgovişte Agriculture. 2023;15:58-62. doi:10.2478/agr-2023-0019

Huang S, Liu S, Wen P, et al. Effects of Different Water Contents on the Quality Characteristics of Roasted Large Yellow Croaker (Larimichthys crocea) Fillets. Foods. 2025;14. doi:10.3390/foods14091638

Farida I, Samanta PN, Maulana H. Evaluasi Mutu Nutrisi dan Organoleptik Tepung Ikan yang Berasal dari Bagian Tubuh dan Kepala Ikan Lemuru. Jurnal Peternakan. Published online 2024. doi:10.24014/jupet.v21i1.22683

Devadawson C. Effects of Different Type of Processing Methods on Biochemical Changes of Skipjack Tuna Katsuwonus pelamis. International Journal of Science and Research (IJSR). Published online 2021. doi:10.21275/sr21330122034

Anggraini P, Asikin AN, Kusumaningrum I. Pengaruh Konsentrasi Asap Cair terhadap Karakteristik Kimia dan Organoleptik Ikan Baung (Mystus gulio) Asap. Media Teknologi Hasil Perikanan. Published online 2022. doi:10.35800/mthp.10.1.2022.35361

Sharma B, Keast R, Liem DG, et al. Plant-protein isolates and flavour perception: Understanding mechanisms and strategies to balance flavour retention and release. Food Chem. Published online 2025:145815. doi:10.1016/j.foodchem.2025.145815

Nur R, Wahab I, Kore J, Saibi N. The Effect of the Use of Type of Wood on the Appearance and Flavor of Smoked Skipjack. East Asian Journal of Multidisciplinary Research. Published online 2023. doi:10.55927/eajmr.v2i1.2512

Cheng YT, Huang P, Lu W, et al. Creating added-value filet product from rainbow trout (Oncorhynchus mykiss) by salting and smoking method: physicochemical and textural attributes. 2023;7. doi:10.3389/fsufs.2023.1153862

Wicaksono AR, Swastawati F, Anggo AD. Fatty acid profile and changes in quality of smoked barracuda fish (Sphyraena jello) with different concentrations of liquid smoke. Food Res. Published online 2023. doi:10.26656/fr.2017.7(s3).7

Vazquez T, Tello E, Peterson D. Identification of the Key Aroma Compounds in Condensed Hardwood Smoke. Molecules. 2025;30. doi:10.3390/molecules30030720

Feng H, Timira V, Zhao J, Lin H, Wang H, Li Z. Insight into the Characterization of Volatile Compounds in Smoke-Flavored Sea Bass (Lateolabrax maculatus) during Processing via HS-SPME-GC-MS and HS-GC-IMS. Foods. 2022;11(17):2614. doi:10.3390/foods11172614

Zhao Y, Xu X, Zeng M. Research progress of fishy odor in aquatic products: from substance identification, formation mechanism, to elimination pathway. Food Research International. Published online 2023. doi:10.1016/j.foodres.2023.113914

Chen L, Zeng W, Rong Y, Lou B. Characterization of taste‐active compositions, umami attributes and aroma compounds in Chinese shrimp. Int J Food Sci Technol. Published online 2021. doi:10.1111/ijfs.15304

Odoli C, Oduor-Odote P, Arason S. The influence of lipid content and pretreatment methods on protein conformation in fish (capelin, Mallotus villosus) during smoking and drying. Food Sci Nutr. 2019;7:1446-1454. doi:10.1002/fsn3.980

Altan CO. A Comparative Study on Quality and Nutritional Values of Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) Treated by Liquid Smoke and Traditional Smoking Methods. Journal of anatolian environmental and animal sciences. 2025;10(5):652-661. doi:10.35229/jaes.1744969

Qiu Y, Zhou Y, Chang Y, et al. The Effects of Ventilation, Humidity, and Temperature on Bacterial Growth and Bacterial Genera Distribution. Int J Environ Res Public Health. 2022;19(22):15345. doi:10.3390/ijerph192215345

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Published

2026-08-07

How to Cite

Cahyono, E., Ijong, F. G., Sambeka, Y., Tanod, W. A., Rieuwpassa, F. J., Ansar, N. M. S., & Batafor, Y. M. J. (2026). Holistic Quality Assessment of Traditional Smoked Fish from the Sangihe Islands: Proximate, Sensory, and Microbiological Profiles . Journal of Nutrition Explorations, 4(3), 430–443. https://doi.org/10.36568/jone.v4i3.743

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